Using reverse-complement or just complement of SILVA database to filter rRNA from metatranscriptomics
I want to use SILVA database to filter rRNA from metatranscriptomics.
The nucleobases in the SILVA fasta file are A, U, C and G. Therefore, before indexing the SILVA fasta file, should I get the reverse-complement or just complement of the SILVA fasta file?
Thanks in advance!
Mort
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