Adding repeats in a genome fasta at a particular location without messing up the annotations?
I want to add a bunch of expanded repeats in a genome fasta file, for eg. 100 ATTs at a particular location eg Chr1-1:2. How do I that and at the same time update the corresponding gtf or gff3 file bioinformatically so that the corresponding genome annotations remain accurate? I can manually add 300 to each value in the annotation file, but I believe someone will have a smarter way to resolve this.
thanks!
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Your plan sounds quite reasonable. The one caveat is adding tandem repeats may actually _change_ the underlying annotations, but I'm assuming that you know that and that it isn't important for your analysis.