Manually adding genome annotation to annotated file
Hi @ll!
With funannotate I have predicted the annotation for a reference genome of a species that does not have a previous annotation and saved this annotation in a GFF3 file. Now, I have found a recent publication with a functional annotation for 5-10 DNA sequences/proteins of the same species, such as:
physalysin_3 MKGLLLLLSVAVAAVSGCQYSDWSLSFDSAGLSRCDGYNQVIRGFFRSYPRGGRDD [...]
physalysin_3 ATGGGCACATTACGTCGATTTGGAGTTTTCGGACTTTTTTGTTTCTTTCATTTAGCAGCTAGTAATCCTTCCAA [...]
Is there a way to manually add a few (approx. 5-10) such (known) protein/DNA sequences to a genome annotation somehow?
Thank you for your help!
Joe
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Kevin Blighe
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joelepaul •
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Yes, determine their position in the genome and then just manually edit the pre-existing GFF file, and then Bob's your uncle.
Kevin
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