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Tools for whole genome alignment sequence conservation scoring?

Dear Community,
I am curious what tools people are using for multiple whole genome alignments and sequence conservation scoring. I'm looking to compare the conservation of enhancer regions across multiple species similar to this figure. I have looked at using mVista but am turned off that this softawre does not accept MAF files and requires a very specific file format. Could anyone point me to a good workflow, alternative to vista, or tool to make using vista less painful?
Thanks in advance!

genome vista whole wga synteny alignment

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