Using TCGA RSEM data to calculate isoform expression
Hello everyone,
I have download the TCGA RNAseq RSEM data about isoform expression. I would like to check which one of the isoforms of my gene is the one expressed the most. Can I directly use the RSEM values to conclude that ? Or should I do some sort of normalization before the statistical analysis?
• 1,030 views
•
link
updated
by
wwu222 •
•
written
by
bnfrmtcs •
•
written
by
bnfrmtcs •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Summarizing transcript level annotation from Trinity de novo assembly to gene level annotation
written by MB •
-
Accessing TCGA Isoform Expression data
written by Jack •
-
How to select the best isoform for a differential expressed gene in Trinity?
written by upendrakumar.devisetty
-
How to find significantly mutually exclusive expressed genes in TCGA
written by Mike
-
Hypothesis testing for isoform level TCGA RNA-seq expression data
written by deepak18 •
-
RNAseq differential expression analysis with only a set of genes
written by demoraesdiogo2017 •
-
Performing differential expression study on isoforms
written by glady
-
Can PAM50 classification performed on TCGA-BRCA RNASeq RSEM data be used with RNASeq FPKM normalize…
written by manali.rupji •
-
Differential isoform expression analyses
written by firestar
-
TCGA: What are mRNA expression z-scores? Does TCGA have mRNA expression from controls?
written by komal.rathi
Hello, I think you can compare between isoforms using TPM values. By the way, may I ask where did you download RSEM isoform expression data for TCGA samples? Thanks!