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How can I load numerous files from a config file in Snakemake? Is it worth it ?

Hello there!

A few days ago I started using Snakemake for the first time.

Mainly I want to use fasterq-dump to download a big number of files from NCBI and I do it like this:

sra = []

with open("run_ids") as f:
    for line in f:
        sra.append(line.strip())

rule all:
    input:
        expand("raw_reads/{sample}.fastq", sample=sra)

rule download:
    output:
        "raw_reads/{sample}.fastq"
    threads: 8
    params:
        "--split-spot --skip-technical"
    log:
        "logs/fasterq-dump/{sample}.log"
    shell:
        """
        fasterq-dump {params} --outdir /home/snakemake/raw_reads {wildcards.sample} -e {threads}
        """

This is working, but:

  1. How can I load the samples from a configure.yaml file instead. Now I have and external txt file with a list of samples and I read it with python
  2. Is it worth it? Will make my script faster if I load the samples from a configure.yaml?

Thanking you in advance!

snakemake
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updated by seidel seidel • written by blackadder • blackadder

1 answer

Presumably you would put your sample names in the config.yaml file:

SAMPLES:
  - "sample1"
  - "sample2"
  - "sample3"

and then reference it in your input:

configfile: "config.yml"

rule all:
    input:
        expand("raw_reads/{sample}.fastq", sample=config["SAMPLES"])

But I can't imagine it would have any effect on the speed of your process, as certainly python reading a txt file or a config file is not the slow part of a script. If you have a good text file method, that seems simpler than formatting your sample names for yaml in a config file. On the other hand, a config.yml file is more formally tied to a Snakemake file - so I suppose it's up to how you like to organize things.

• 0 views • link
written by seidel seidel

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