Forum: chimeric vs unaligned reads
Hello,
I'm trying to obtain the chemic alignments from a BAM file that originally was generated by STAR and that only has a list of unaligned reads at the end of the file (so no SA tag).
If I convert that BAM file back into a fastq file with samtools and then rerun STAR with --chimOutType WithinBAM, will I label some of these unmapped reads as chimeric ones?
Is there a risk of loosing information/reads by doing so? Unfortunately I do not have the original fastq files and I'm trying to find a way around.
Thank you!
Best, Andrea
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