This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Why do annotating the same functionality with different databases produce different results？

Hello,

I made functional annotation associated with nitrogen metabolism for metagenomic data using KofamScan(https://www.genome.jp/tools/kofamkoala/) ，NCyc（https://github.com/qichao1984/NCyc）and eggnog-mapper. But these results are significantly different. For example, the ORFs that are annotatated as gene nirK using three methods are different. Why is it so?

Thank you.

kegg annotation eggnog metagenomic
• 1,506 views • link
updated by Jean-Karim Heriche Jean-Karim Heriche • written by Xudong Li • Xudong Li

1 answer

Different resources may have different notions of what a gene is (they may use different references), they use different sources of information and collect and aggregate the information in different ways (for example by having different requirements/confidence levels). The result is that different databases contain different annotations.

• 0 views • link
written by Jean-Karim Heriche Jean-Karim Heriche

Log in to answer this question.

More posts like this