DESeq2 Not full Rank - Age & Medication
Hi. I've a data matrix which looks like this:
I'm trying to carrying out DGE analysis controlling for age and medication so I used the following:
design(ddsMF)<-formula(~age+medication+group)
ddsMF<-DESeq(ddsMF)
And end up with the error, "Full model matrix is less than full rank."
I saw the vignettes on the DESeq2 tutorial but am not sure if they would work here given the large age range. Any advice would be most welcome.
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Devon Ryan
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Anand •
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Odds are good that you have a typo somewhere in your design matrix. Make sure group and medication are factors with only 2 levels and that age is numeric, rather than a factor. Otherwise the model matrix should be full rank.
Fixed! Thanks Devon.
For future people with the same issue, can you mention exactly what was wrong?