I have a rather primitive question
After importing my sequence alignments to either "Geneious" or "BioEdit", I cannot export them in the same 60pb per line format, does anyone know how to do that to avoid the manual line adjusting? Alternatively if there is a web-based line width convertor (e.g. 80 to 60 or 100 to 60 or infinite to 60,..) to solve the problem quickly, please let me know
4 answers
The FASTX-Toolkit is a collection of command line tools for Short-Reads FASTA/FASTQ files preprocessing. You may install them and use them locally.
Alternatively, its ability to modify FASTA width is available on Galaxy under the name "FASTA Width formatter".
This one might do it, not sure:
https://www.ebi.ac.uk/Tools/sfc/emboss_seqret/
or in Galaxy there is a tool called: FASTA Width formatter
unix command fold can format lines on defined length
reformat.sh from BBMap suite. Set NN to desired number.
reformat.sh -Xmx2g in=your.fa out=reformat.fa fastawrap=NN
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