Hi, I had a 7 different genomes (fish) with 40X genome coverage, sequenced by Nextseq-500. 1. I used 3 de novo assemblers (Abyss(21,33,44,77), Spades(21,33,44,77), Velvet(31)) …

Hi guys I have a serious problem with my data I want to do the Denovo assembly genome with size genome(AFTER TRIMM):2.6 GIG and coverage …

Hello I have question about validation assembly, can i validate assembly with T.A Cloning? i can't find similar research in this field, if anyone can …

Hi I am working on viral genome and the reads i generated is from ion torrent method. After performing denovo assembly using trinity tool i …

Hi all, I'm analysing RNA-Seq data to finally calculate RPKM value. First I did denovo assembly of reads using Trinity assembler. Then using Trinity output …

Hi How to determine the optimal k-mer value using velvet software for denovo genome assembly? Thanks

Hi, I have did a denovo assembly on RNA seq data. On mapping the contigs there are few genes whose functions are unknown. Is there …

<p>I have used the columbus extension of velvet to do a <strong>templated</strong> assembly, which, as far as I can tell, was successful. Until someone pointed …

<p>Hello.</p> <p>I was working on a pipeline for genome assembly and I have used the human paired-end NGS data.</p> <p>I want to compare my denovo …

Hi Community users, I have single-end reads fastq file. I have generated denovo assembly using velvet/oases. Now I need to check how good is my …