problem with Trinity command run in denovo transcriptome assembly
I tried to run my Trinity command for the assembly but this is the error I've been getting .. please help let me know where did I go wrong. I'm new to this analysis field.
Error, cannot locate Trinity-specific tool: ParaFly in the PATH setting: /usr/local/bin/trinity-plugins/BIN:/home/aliviya21/anaconda3/bin:/home/aliviya21/anaconda3/condabin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin:/usr/games:/usr/local/games:/snap/bin:/home/aliviya21/Downloads/bowtie2-2.4.1:/home/aliviya21/BIONIVID/trinityrnaseq-v2.13.0, be sure to install Trinity by running 'make' in the base installation directory
Thank you in advance
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Use
condato install.conda create -n trinity trinityand thenconda activate trinityshould do it. Reference: Creating workflows with snakemake and condaI did the same prior, created an env and tried running the program. This is the error I've been getting the same one with or without an environment. . Error, cannot locate Trinity-specific tool: ParaFly in the PATH setting: /usr/local/bin/trinity-plugins/BIN:/home/aliviya21/anaconda3/envs/trinity/bin:/home/aliviya21/anaconda3/condabin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin:/usr/games:/usr/local/games:/snap/bin:/home/aliviya21/Downloads/bowtie2-2.4.1:/home/aliviya21/BIONIVID/trinityrnaseq-v2.13.0, be sure to install Trinity by running 'make' in the base installation directory