Hello,

I used DESeq2 to see which ASVs were differentially abundant between different treatments on 16S metabarcoding data. I now want to plot the relative abundance (in %) of those ASVs.

However, I am unsure which data would make to most sense to plot... should I use:

• the raw reads table transformed to %
• the rarefied reads table transformed to % (which I used for beta-diversity)
• the normalized or transformed reads count table (rlog or vst) from DESeq2

Wouldn't the first two options represent the original data better?

I am sorry as this is probably a very basic question, I just can't figure it out!

Thank you very much !

Marion