How to interpret bimodal distribution of GC-content for RNAseq and can it be remedied ?
A colleague of mine have got the following distribution of GC-content for RNAseq.
How to interpret bimodal distribution of GC-content for RNAseq ? Does it mean some contamination ? Is there any method to remedy ?
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A broad peak usually indicates contamination. To confirm that, you can run fastQ screen.
Start checking for contamination with rRNA.