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RNA-seq normalisation

Hello members, I have downloaded SRA files and converted them to fastq files. I wish to work on RNA-seq normalisation using these fastq files without converting them to read counts. Please let me know how to do normalisation of these files

normalisation rnaseq

2 answers

It sounds like you need to get a basic background first.

RNA Sequencing Data: Hitchhiker’s Guide to Expression Analysis

This countains a basic heads-up on the relevant basics.

GREIN : GEO RNA-seq Experiments Interactive Navigator can give you the normalized read counts after your inputs GSE id.

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