So how to check that whether I have done the analysis correctly or not?
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I have analyased the miRNa and mRNA expression profiles of pancreatic cancer using the genespring software,But how I shall make sure that I have analyed it correctly because results vary among the differents softwares,diff parameters and algorithms used in whole analysis.Is there any way to check?
You answered your own question. The results for a given data set vary between different analysis because you changed the analysis settings.
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You need to be more specific than that.
What do you mean by discordance, what do you consider as being different and why should the results be the same