RNA-Seq featureCounts Results Annotation File
Hello everyone, I have human Rna-Seq data reading by MiniOn.
I aligned with the human reference genome (GRCh38.p13) from that website= https://www.gencodegenes.org/human/ using minimap2. I also downloaded annotation from the same link which is Comprehensive gene annotation(All). My question is why featureCounts results are too low, I controlled with multiqc then it's assigned the only %5. These are summary of counts data
Max. :4.1e+01 Max. :79.0000 Max. :6.6e+01
How to create an annotation file, it should be correspond specific region or not ?
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santos48 •
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