Cut&Run fragment length
Hi,,
I run Cut&Run on four samples and when I assessed the quality metrics I found that most of the fragment length of the reads are more than 120bp. Based on cut&run paper, fragment length should be less than 120bp to consider a good a data set. My conclusion is that the antibodies used failed to bind to the genomic DNA maybe because is not specific to the targeted regions. Is anyone has another explanation for getting longer fragment length.
Thanks
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Species matching IgG controls in CUT&RUN experiment
written by kevin.stachelek •
-
Discrepancy Between Fragment Size Distribution and MACS3-Predicted Fragment Length in ChIP-seq Data…
written by christoph.neu •
-
Question about different lengths of fragments in ATAC-seq
written by Chris •
-
Getting coordinates of unmapped regions and regions that map to them
written by ja569116 •
-
Get the percentage of fragment length that is less than 120bp
written by munaj86 •
-
Bam file metrics
written by Sabeen •
-
GC Bias Correction
written by finswimmer
-
Nucleoatac - extending broadPeak regions
written by BioinfGuru
-
How to view reads/parts of reads that lay outside of reference from bam format
written by tobias221188 •
-
Correlation Between Intron Length And Presence Of Intronic Tfbs?
written by 14134125465346445
