Cell Ranger low "Fraction Reads in Cells"
Hello.
I have a question for the Cell Ranger.
I analyzed some uncommon species (Zebrafish and nicotiana tabacum) using Cell Ranger 6.0.0.
I confirmed web_summary.html for these samples and all of these were identified low "Fraction Reads in Cells".
All parameter for Cell Ranger was used default (no --force-cells) and target number of cells was 10,000.
10x Genomics commented like this (https://kb.10xgenomics.com/hc/en-us/articles/360003919491-How-to-interpret-the-Fraction-Reads-in-Cells-metric-), but I think experiment had no problem.
If you don't mind, could you tell me how can I solve this problem?
Sincerely.
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