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NCBI reported coverage from long and short reads

Hi there,

I was wondering, for the purpose of NCBI genome submission, can I add together the two coverages I calculated from Illumina Hiseq and Pacbio sequencing? E.g. Illumina coverage = 450, Pacbio coverage = 150, Total coverage reported to NCBI = 600X

Can this be done?

Thanks! Morgan

pacbio ncbi genome illumina
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written by Morgan S. • Morgan S.

Hi Morgan, How did you calculate the coverage for long read data? Did you use a tool for it?

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written by Arton • Arton

Hi Arton,

I used this equation from the Illumina website. C = LN / G

C stands for coverage G is the haploid genome length L is the read length N is the number of reads

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written by Morgan S. • Morgan S.

1 answer

If you are calculating gross coverage in terms of base pairs present in the sequence then yes.

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written by GenoMax GenoMax

Yes, this is what I did. Thanks so much for your quick reply!!

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written by Morgan S. • Morgan S.

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