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Determining RNAseq background noise

Hi,

I am interested to see if read counts of three RNAseq samples (same tissue) are above background noise. I was wondering whether there is a way to approach this systematically rather than arbitrarily setting a count threshold and remove transcripts below it. For example would it be possible to determine a distribution for the background noise and compare each transcript's counts against it?

Many thanks:), Marcos

rna-seq rnaseq rna-seq rnaseq rna-seq

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