Compatilibity between human37 and human38 gtf
Hi,
I aim to make miRNA:target prediction and later I will look for the target gene expression levels from the available count matrix. Count matrix is published in which authors used Ensembl GRCh37v75 transciptome for alignment.
For target prediction, I have to obtain 3UTR fasta file first. Should I also use Ensembl GRCh37v75 gtf to obtain 3UTR fasta? Is it a problem if I use Ensembl GRCh38 gtf?
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WouterDeCoster
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asumani
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Yes. The coordinates are not guaranteed to match.