This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Using RPKM to compare gene expression between samples

Hi all, wondering if normalisation using log2(rpkm + 1) is suitable for comparing gene counts between samples and groups. I see this quite often in pubs, e.g.:

Screen-Shot-2021-04-26-at-4-42-52-pm

But I came across this, which says RPKM is for "gene count comparisons between genes within a sample; NOT for between sample comparisons or DE analysis"

Thanks!

rnaseq normalization rpkm

1 answer

The pubs that do things like that are doing things incorrectly. If you're comparing between samples, there are many programs written for that purpose (e.g. deseq2, sleuth, edgeR, etc.)

Log in to answer this question.