Using RPKM to compare gene expression between samples
Hi all, wondering if normalisation using log2(rpkm + 1) is suitable for comparing gene counts between samples and groups. I see this quite often in pubs, e.g.:
But I came across this, which says RPKM is for "gene count comparisons between genes within a sample; NOT for between sample comparisons or DE analysis"
Thanks!
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The pubs that do things like that are doing things incorrectly. If you're comparing between samples, there are many programs written for that purpose (e.g. deseq2, sleuth, edgeR, etc.)
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