how to align sequences in multifasta
Hi,
I have a multifasta with 14 sequences. The sequences are from 14 different isolates, but they are all the same gene.
Four of them are the + strand and the other ten are the - strand (which I believe means they oriented differently).
What is the best tool to properly align these sequences and make a NJ tree?
Any help is appreciated!
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GenoMax
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goatsrunfaster •
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goatsrunfaster •
1 answer
any multiple alignment tool should do I think. Eg. Muscle, emma, clustal-omega, ...
you do have to make sure that all sequences are in the same order in the fasta file (most commonly 5'-3' , so start to stop )
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lieven.sterck
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If you need to use a graphical program then MEGA is a good option on PC/Mac.