New Pileup Output
The pileup output description is different than the output I am getting now. Could anyone help me with a definition of each column? http://samtools.sourceforge.net/pileup.shtml
This is a line from my output. It has additional columns when compared against the original description.
1 621 T G 159 159 36 74 g$g$gggggGGgggggggGGggGGGGgGGgGggGGgGGgggGGgggGGgggGGggGggGgGgGgGggGggggGGgG !!!!***A:2223222:32238:8273232237522532222222322222922222322222222233322'!
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See here:
http://samtools.sourceforge.net/samtools.shtml
Note the section under pileup where there are more details given about the extra columns. Also, note the pileup is deprecated and will be soon dropped from samtools, so consider switching to mpileup for variant calling.
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strange, which program did you use?
I used the pileup command. I think that Sean Davis answered my question.