Hi, I'm searching for e.g. 50 sequences in Not redudundat blast database. I want to test program for protein mutation prediction - program tries to estimate if mutation is deleterious or neutral.
Example of analyzed sequence is well known lacI repressor. Blast finds lot of sequences but too much similar. First 50 sequences are almost the same and prediction program has no heterogentity for it's prediction model.
How to find homogous sequences but not the same (I want orthologs). E. g. sequences from another species and little bit different than human LacI protein.
I tried classic blastp. Another way I tried: first run blastp for 2000 sequences and then align these sequences and this alignment get to psiblast as PSSM (-in_msa parameter). Is there other automatic way or parameter settings for Blast+ package to find more distant sequences?
EDIT: Constraint - searching process have to be automatic. It is one of the component of a bigger tool.
•
written
by
onpelikan •
You're looking for a search with an improved sensitivity. Try a profile-based search, e.g. HMMer with pfam.
HMMer returns lot of sequences so I clustered it with cd-hit and this process got the best results for mutation analysis with MAPP program.
You can do with BLASTO blast designed for orthologue search. Try search in eggNOG database or DRSC tool.
Thank you. This is really interesting projects/tools but I need command line program (such as blast+ programs).
Is there please any way to download all sequences in fasta? I can't see anything.
This is another good advice.
1) I need the blast to be automatic process without manual work.
2) I will check the original paper. Thank you.
If you do not want to rely on an orthologous groups database, modify your input set to include diverse sequences by cd-hit (http://weizhong-lab.ucsd.edu/cd-hit/).
This is how protein families were built in the olden days of biocomputing.
This is probably one of the best solution. One possible is let blastp search e.g. 3000 sequences and then obtain 50 representative sequences from cd-hit clustering .
Log in to answer this question.
I would guess you need to define some sort of constraints - i.e. (1) bitscore thresholds, (2) species subset (or a distance) and (3) conserved domain(s), and then see which blast hits will satisfy these.