Error In Galaxy Macs Peak Calling With .Bed Files
I was using Galaxy MACS to do peak calling using a pair of case and control .bed files. I didn't specify any of the parameters. An error occurred: error An error occurred with this dataset: Usage: macs <-t tfile> [-n name] [-g genomesize] [options] Example: macs -t ChIP.bam -c Control.bam -f BAM -g h -n test -w --call-subpeaks macs: error: no such option: --lambdaset
Why do the file names turn out to be .bam? What's the meaning of the error?
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I think the BAM files is only an usage example. Your Galaxy wrapper is not working properly. You should talk to your Galaxy Administrator to get the wrapper in sync with the MACS version. It seems that --lambdaset is not available in the installed MACS version in your Galaxy instance.