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Rna-Seq Rpkm For Coexression By Wgcna

I am new to coexpression network construction. In my hand, i have 89 samples of different treatments from different tissues, and the same tissue treated by the same treatment in different time. like below: treatment: Didymella bryoniae , tissue: leaf

  • leaf at fruit 300 grams stage, before infection
  • leaf at fruit 300 grams stage, 24 hrs after infection
  • leaf at fruit 300 grams stage, 1 week after infection

I want to use these data to construct a coexpression network, how to manage the data for WGCNA input?

network

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