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Where Could Find The 3' Adapter Sequences Of Illumina'S Hiseq 2000
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6.2 years ago
bmechuangye • 20

I have download some sequencing data from NCBI SRA but it doesn't provide the information about their adaptor information. Could someone tell me the 3'adapter sequences of Illumina's HiSeq2000 or where could find them? Thank you !

illumina hiseq • 4.4k views
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13 months ago
Rm 7.8k
Danville, PA

http://seqanswers.com/forums/showthread.php?t=198

From Illumina Website

"Illumina Adapter Sequences Letter: This letter contains Illumina adapter sequences. It is provided for use only with Illumina instruments: Genome Analyzer, HiSeq, HiScanSQ, and MiSeq."

http://supportres.illumina.com/documents/myillumina/6378de81-c0cc-47d0-9281-724878bb1c30/2012-09-18_illuminacustomersequenceletter.pdf

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Thank you! But the Genome Analyzer adapters may different from the HiSeq adapters are different.

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Edited my answer

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Thanks a lot, Rm.

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6.2 years ago
GAO Yang • 250

Send an email to techsupport@illumina.com, they will provide the sequence info you need. But first of all, you need to know which sample prep kit it used. Different kits may have different adaptors.

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Thanks, GAO Yang

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