Bam To Afg
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Sam To Bam
written by freddy •<p>I sequenced a mito genome using paired end method on the mi-seq I mapped all the reads back to reference gnome using <a href='http://bio-bwa.sourceforge.net/'>BWA</a> and …
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How To Sort Sam File
written by Chen Sun<p>I want to process the <a href='http://samtools.sourceforge.net/SAM1.pdf'>sam</a> file created by <a href='http://bio-bwa.sourceforge.net/'>BWA</a>, if it is sorted by the sequence coordinate on reference, it will be …
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Sam To Bam - Loss Of Data Or Just Great Compression?
written by Clare<p>I use picard tools to take a <a href='http://bio-bwa.sourceforge.net/'>BWA</a> alignment <a href='http://samtools.sourceforge.net/SAM1.pdf'>sam</a> file and convert it into a sorted <a href='http://samtools.sourceforge.net/SAM1.pdf'>bam</a> file. Normally this works …
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Can Igv View Blast+ Output File?
written by shl198<p>Hi, I aligned short reads using blast+ and generated tabular file. I also have <a href='http://samtools.sourceforge.net/SAM1.pdf'>bam</a> files generated by aligners, and I can visualize the …
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Removing Phix Data From Ngs Data For Analysis
written by crivenster<p>I have a NGS data containing 95-98% of PHIX data,which i dont need. So after some reading, I used <a href='http://bio-bwa.sourceforge.net/'>BWA</a> to align the reads …
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Error In Converting Sam To Bam By Samtools
written by Raghav<p>hello every one,</p> <p>after mapping,i encountered an error during converting my <a href='http://samtools.sourceforge.net/SAM1.pdf'>sam</a> out file into <a href='http://samtools.sourceforge.net/SAM1.pdf'>bam</a> out put file.</p> <p>[cdac@nbri <a href='http://samtools.sourceforge.net/'>samtools</a>-0.1.19]$ <a …
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How To Extract Consensus Sequence Of Mapped Regions From A Bam File?
written by lin.barnum •<p>I would like to extract consensus sequence of only the mapped regions from a <a href='http://samtools.sourceforge.net/SAM1.pdf'>BAM</a> file. I have a <a href='http://samtools.sourceforge.net/SAM1.pdf'>bam</a> file which contains …
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When Using Cuffdif Can I Combine Bam Files Obtained From Different Experiments?
written by narges •<p>Hi all,</p> <p>I wanted to ask whether the order of genes in two different <a href='http://samtools.sourceforge.net/SAM1.pdf'>BAM</a> files when running cuffdiff is important or not? To …
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Sam To Bam Conversion Reference Fasta File
written by Atom Smasher •<p>Hello,</p> <p>I had some data in "eland_export.txt" format and I want to convert it to the <a href='http://samtools.sourceforge.net/SAM1.pdf'>SAM</a> format first and then later to the …
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Soap Result Converted To Bam Not Working In Samtool
written by Sarwar •<p>I converted the output of soap2 which is map file to <a href='http://samtools.sourceforge.net/SAM1.pdf'>sam</a> file using map2sam.pl and then converted this <a href='http://samtools.sourceforge.net/SAM1.pdf'>sam</a> to <a href='http://samtools.sourceforge.net/SAM1.pdf'>bam</a> …