Tophat: Option --Bowtie Not A Unique Prefix
This is a new problem for me: tophat: option --bowtie not a unique prefix, when I try to run tophat (ver 2.0.6) with bowtie 1.0.0 installed
tophat -p 4 --bowtie \ --fusion-search \ -z 0 \ --library-type fr-unstranded \ --GTF $GENOME.gtf --read-mismatches 3 \ --segment-mismatches 2 \ --segment-length 25 \ --bowtie-n \ -o outputFolder \ input_DIR/file1.fastq.gz
It's complaining tophat: option --bowtie not a unique prefix, I don't understand. Could someone help me? thanks
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You need to specify --bowtie1
not --bowtie
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Do you mean --bowtie1 ? There is no plain --bowtie argument.