Local Alignment Of Rna-Seq Data
Hi,
Anyone knows a good tool to align RNA-Seq data in local alignment mode ? The tool has to be splice-aware. Example (in red: aligned nucleotides , in gray, soft-clipping )

Thanks
N.
rna-seq
local
alignment
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Nicolas Rosewick
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I don't actually understand what you mean by local alignment mode..
But I think Tophat2 does the mapping keeping in mind the splice variants.
You can use subread too http://subread.sourceforge.net/