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What Is The Difference Between Rna-Seq And Transcriptome Sequencing?

Hi all,

I guess they are different.
Are they different?
If so, what is the difference?

Thanks in advance.

rna transcriptome
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updated by Alex Alex • written by toshnam toshnam

3 answers

I think these are synonyms. RNA-seq is a specific means to measure the state of the transcriptome.

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written by David Quigley David Quigley

+1 Nice link, and it is always tricky to stick your head out by questions where there might be some semantic nuance that you were not aware of.

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written by Chris Evelo Chris Evelo

There is a very nice [?]description in Wikipedia[?]. There are a few different technologies and approaches but, like David said, the two terms are indeed synonyms as long as you mean whole transcriptome sequencing.

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written by Chris Evelo Chris Evelo

I think RNA-Seq is more wide term. RNA-Seq includes transcriptome sequencing (polyadenylated RNA), "all" RNA sequencing (include rRNA and non-coding RNA) and variant for various non-coding small RNA sequencing. The main difference in a method of probe preparation.

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written by Alex Alex

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