Hi all, I hope some expert is there with pdb format. I have small motif with in pdb format. inside the pdb file i want to change Amino Acid residue into a user define name. I did that using awk and i found that orginal file opens in chimera, jmol etc but the changed file is not opening. If i manually replace AA residue then it can works like orginal motif. Please help me in finding the error in awk edited file comparing to orginal pdb file for example:

Orginal file

ATOM 1243 N TYR B 105 65.157 40.402 -13.263 1.00 13.49 N
ATOM 1244 CA TYR B 105 64.938 40.910 -11.922 1.00 13.31 C
ATOM 1245 C TYR B 105 64.747 39.748 -10.950 1.00 14.10 C
ATOM 1246 O TYR B 105 65.292 39.756 -9.849 1.00 14.27 O
ATOM 1247 CB TYR B 105 63.710 41.804 -11.884 1.00 10.62 C
ATOM 1248 CG TYR B 105 63.439 42.357 -10.511 1.00 11.78 C
ATOM 1249 CD1 TYR B 105 63.921 43.597 -10.128 1.00 11.65 C
ATOM 1250 CD2 TYR B 105 62.682 41.640 -9.598 1.00 14.01 C
ATOM 1251 CE1 TYR B 105 63.648 44.113 -8.869 1.00 13.06 C
ATOM 1252 CE2 TYR B 105 62.401 42.149 -8.338 1.00 15.13 C
ATOM 1253 CZ TYR B 105 62.882 43.386 -7.981 1.00 13.16 C
ATOM 1254 OH TYR B 105 62.548 43.905 -6.748 1.00 14.20 O
ATOM 1255 N GLY B 106 63.959 38.758 -11.352 1.00 13.40 N
ATOM 1256 CA GLY B 106 63.744 37.615 -10.491 1.00 14.83 C
ATOM 1257 C GLY B 106 65.044 36.864 -10.231 1.00 15.24 C
ATOM 1258 O GLY B 106 65.287 36.428 -9.107 1.00 13.96 O

Edited Using AWK

ATOM 1243 N A01 B 105 65.157 40.402 -13.263 1.00 13.49 N
ATOM 1244 CA A01 B 105 64.938 40.910 -11.922 1.00 13.31 C
ATOM 1245 C A01 B 105 64.747 39.748 -10.950 1.00 14.10 C
ATOM 1246 O A01 B 105 65.292 39.756 -9.849 1.00 14.27 O
ATOM 1247 CB A01 B 105 63.710 41.804 -11.884 1.00 10.62 C
ATOM 1248 CG A01 B 105 63.439 42.357 -10.511 1.00 11.78 C
ATOM 1249 CD1 A01 B 105 63.921 43.597 -10.128 1.00 11.65 C
ATOM 1250 CD2 A01 B 105 62.682 41.640 -9.598 1.00 14.01 C
ATOM 1251 CE1 A01 B 105 63.648 44.113 -8.869 1.00 13.06 C
ATOM 1252 CE2 A01 B 105 62.401 42.149 -8.338 1.00 15.13 C
ATOM 1253 CZ A01 B 105 62.882 43.386 -7.981 1.00 13.16 C
ATOM 1254 OH A01 B 105 62.548 43.905 -6.748 1.00 14.20 O
ATOM 1255 N A02 B 106 63.959 38.758 -11.352 1.00 13.40 N
ATOM 1256 CA A02 B 106 63.744 37.615 -10.491 1.00 14.83 C
ATOM 1257 C A02 B 106 65.044 36.864 -10.231 1.00 15.24 C
ATOM 1258 O A02 B 106 65.287 36.428 -9.107 1.00 13.96 O