I am new to this world of Bioinformatics and I just learnt what is DNA sequencing. I am a computer scientist and I wish to try out some of my own algorithms on the DNA. Now where to get DNA in a text file. I mean I wish to have the sequence like ATGTCC .... which has billions of these letters (nucleotides) in a text file. Can somebody help me. I hardly understand the different websites which i am led to when I google. It might be a a really dumb question. But help, sarcasm and criticism are appreciated!
3 answers
Ensembl keep all our sequences here:
ftp://ftp.ensembl.org/pub/current_fasta
We've got gene, genome and protein sequences in FASTA format for a variety of species.
You can go to genbank and download any gene sequence you want or go to ucsc and download the human genome.
Best would be : NCBI [http://www.ncbi.nlm.nih.gov/] and find "a gene/ protein name" say for example- albumin. And then keep clicking and exploring to find out the ATGC (for genes) and DDVLI (for proteins encoded by upto 20 such amino acids!)..... and get the "FASTA" formatted files onto a Notepad ! REST all falls in place and assured ! Thanks,
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If you are interested, you can start with a few ppts like these,
http://staff.aub.edu.lb/~webbic/tutorials/databases.ppt
www.sanbi.ac.za/wp-content/uploads/IntroToDatabasesDay1.ppt
www.bioinf.org.uk/teaching/c40/ppt/intro_databases.ppt
some of them might be a bit outdated on statistics for databases, but will give you some useful info.
Instead of directly trying on human sequence try to do it on smaller and simpler genomes of microbes, available here => http://www.ncbi.nlm.nih.gov/genomes/MICROBES/microbial_taxtree.html