Bacteria Intergenic Regions Rna Depleted

I was wondering if I need to get the data (differential expression) in the intergenic regions of bacteria do I need to work on ribo- depleted/ ribo zero library generated data or not?

Thanks

rna-seq

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updated 11.4 years ago by Asaf

• written 13.3 years ago by kanwarjag

1 answer

Yes.

Otherwise ~90% of the reads will be of rRNAs. Make sure you don't throw away short RNAs which will probably enriched with IGR encoded RNAs.

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written 11.4 years ago by Asaf

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