I want to calculate the conservation of a segment of RNA sequence (about 50 nt),. I tried phastCons but failed, because I do not know …

Does anyone know how to calculate the substitution rate (e.g. how many substitution per site) for a pair or a bunch of nucleic acid sequences? …

<p>I have tried using BioPython's melting temperature calculator to compute the Tm for 10mer RNA-RNA hybridizations. However, I was wondering about its accuracy. Does anybody …

<p>How to generate RNA multiple sequence alignment based on the secondary structure that is already known of one or several sequences? Which programme should I …

<p>Hi all,</p> <p>I am going to use RNAplot (in Vienna RNA package) to draw a figure of my RNA secondary structure. I however, I do …

<p>Hi, does anyone know whether the input tree for PAML codeml analysis should be got based on codon alignment or amino acid alignment? Thanks!</p>

<p>Since it often takes a long time to calculate the dN/dS value of a big tree, how can I calculate the value only for some …

<p>Hi everyone,</p> <p>Does anyone know how to calculate the number of synonymous sites for a certain sequence? I know the principles, but I don't know …

<p>Hi! I just found this site from reddit, and thought I'd ask this question here as well. I have a large number of DNA primer-target …

<p>Hi All,</p> <p>I'd like to know whether a point mutation in my protein structure would increase/decrease its stability and how much. Is it possible to …