Expression Studies Of Alternative/Splice Variants Of A Gene
hi is there any sophisticated method (other than Real Time PCR) to study the expression of splice variants of a gene which differ by one or two codons
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I'm not sure what you are really asking ("sophisticated method" is a bit vague), but there are several approaches to study changes in splicing using RNA-seq data, such as:
- DEXSeq: R | Publication
- BitSeq: Python | R | Publication
- Cufflinks: Software | Publication
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On StackOverflow this question would be closed as too open and vague. Suggest you improve it to avoid the same fate.
Elobarate your question...
I was studying the relative abundance of two splice variants (mRNA) under different stress conditions by qPCR. These two splice variants differ by one codon (revealed by RNA-seq data).