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What is the best method for selecting significant genes in one class expression profiles?

I have one class gene expression which I want to find the most significant genes to do more precise analysis.

I saw somewhere which had defined a threshold and selected those gene which their expression were greater than the threshold. I am wondering is there any other method to do such analysis more precisely?

Thansk.

significance analysis gene expression
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updated by Ram Ram • written by Na Sed Na Sed

What is a "significant gene" in your experiment? What is the biological question you are trying to answer?

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updated by Ram Ram • written by Sean Davis Sean Davis

I am going to analyze miRNA-mRNA interactions through their expression profiles. In this regard, the number of mRNAs as well as miRNAs are too much, so I want to decrease the number of both and keep just those which have ,ore variability.

Thanks.

N

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written by Na Sed Na Sed

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