External Cgi Script
I have files: <123.cgi> , <gene.fasta> and <file.html> in my "c:\users\desktop" folder.
<123.cgi> contains code to handle gene sequence file <gene.fasta> and calculates some genomic parameters.
What should be the code in <file.html> to make this 123.cgi execute and print the results on a web page without anybody knowing the CGI code. Thank you in advance.
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That is not an HTML problem, at least not primarily. You need to study the configuration manual of your Web server (and these are very different for different servers) and set up the directory attributes. With the information you posted, this question cannot be answered.
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Closed, off topic. It is a general web-server configuration problem.