I have a set of gene of interest with ensembl Gene ids and I want to do functional annotation for each and to categorize them based on function. I am interested to do other than doing with blast2GO. I have tried with DAVID tool, but I don't find any functional annotation for my set of genes. Can I try with eggNOG?. Any suggestion for using better tool for functional annotation.
2 answers
You might find BioMarts ID conversion tool very handy. You can get GO terms and much more.
http://central.biomart.org/converter/#!/ID_converter/gene_ensembl_config_2
also check out Amigo:
BioMart is a useful tool for this, as mentioned.
The Ensembl Core Perl API can also be used to retrieve GO terms.
Installation instructions are here:
http://www.ensembl.org/info/docs/api/api_installation.html
Modules are here:
http://www.ensembl.org/info/docs/Doxygen/core-api/index.html
Specifically, you would want the ontologyterm adaptor methods.
If you have more questions about how to get GO terms for Ensembl transcripts, email us at helpdesk@ensembl.org
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