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Convert coordinates from cDNA fasta to genome fasta

Hello kind folks,

I've mapped some long read sequencing data to mouse cDNA fasta. I would like to see how the coverage is changed around splice junctions, but I don't know how to convert my splice junction coordinates (which are relevant only to genomic coordinates) to transcriptome coordinates.

Does anyone have any idea how to proceed?

The genome fasta, cDNA and genome annotation (splice junctions) are all from Ensembl.

Thanks, AJ

rna-seq cdna gnome fasta

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