I have used the following line of code before to convert my .csv file to GRanges:

#Converting csv to GRanges 
gr <- makeGRangesFromDataFrame(Test, keep.extra.columns=TRUE)

Weirdly enough, this line is still working for an older file but no more for the new file. Here is the error that I see:

Error in .Call2("C_solve_user_SEW0", start, end, width, PACKAGE = "IRanges") :
  In range 20498: at least two out of 'start', 'end', and 'width', must
  be supplied.

I made sure the columns are same as the old file (that works).