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Annotate PBMC scRNA-seq data

Hi, all! I am dealing with this dataset https://support.10xgenomics.com/single-cell-multiome-atac-gex/datasets/1.0.0/pbmc_granulocyte_sorted_3k. I need to identify the cell type for each cell then do unpervised clustering etc. Do you have any suggestions?

rna-seq

1 answer

You can check this tutorial for working with a scATAC and scRNA-seq dataset where they also use PBMC data: https://satijalab.org/signac/articles/pbmc_vignette.html

Thank you very much! I will have a try.

So what can I do if I only want to use scRNA-seq data to do annotations?

There are multiple discussions on this site about scRNA-seq cell annotation.

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