Sequence length distribution after adapter trimming using trimmomatic
I have a chipseq library with sequence distribution of mean 150 from fastqc. After adaptor trimming, the distribution became from 35-149. quite wide.
I am getting acceptable NSC but RSC < 0.1, quality tag < 0 from Phantompeakqualtools tools. My guess is it is coming from varying fragment distribution.
Is there any way I can save this library?
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Poly-G and Missing data Novaseq X vs Novaseq 6000
written by Butterfly •
-
Rsubread with unexpected read order results
written by thomas.heigl.ibk •
-
DiffBind issue in identification of differentially enriched peaks between two conditions
written by accibio •
-
Varying read length distribution after running Trimmomatic
written by basuanubhav •
-
Paired end sequencing in case of a short biological insert
written by Aspire
-
FastQC & trimming - RNA seq data
written by dietrima •
-
Low percentage of Illumina reads retained after trimming with Trimmomatic
written by Bea •
-
I got extremely low alignment rate running HiSAT2 and Tophat2
written by oghzzang •
-
BWA-MEM Vs BWA-ALN
written by SOHAIL
-
Tutorial: InsideDNA: Analysing transcription factors with ChIP-Sequencing data: a full bioinformati…
written by shilparaopradeep •
