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miRDeep2 discovers different novel miRNAs in each run

Hi All,

I had a simple but important observation only recently, that when you run same sample in miRDeep2 multiple times, around 15 out of 90 miRNAs are unique to each run. That is a big number.

Can anyone guide me if miRDeep2 is meant to be stochastic in nature, i.e. this behaviour is expected and why?

Thank you, Rituriya

mirdeep2 novel mirna mirna

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