Hello everyone I came from statistics background now I learning WGCNA analysis for the micro array data by using R programming. When I used bolckwisemodule function I got around 8 modules. I'm unable to find out what are the significant genes from each module please help me for finding the significant genes.
1 answer
WGCNA is not a technique for finding significant genes. Instead it is a clustering alogrithmn that clusters genes with correlated gene expression across samples together. Modules of genes are created using a dynamic tree cutting approach which doesn't have a hard threshold. That is, there is no statistical significance (in the p-value sense) attached to a gene being associated with a module. Are you looking to extract the genes that belong to each module?
If you want to find significantly different genes between two conditions I recommend using an algorithm designed for that purpose. The most common is limma.
Log in to answer this question.
Kindly please help me how to find the significant genes for the micro array data