Hello everyone, I have a hundred genomes and I'm looking for repeated regions along these assemblies.
First of all, I thought of using RepeatModeler, only the most familiar among you know that it is not at all optimized software that requires a lot of resources and calculation time, which is therefore not feasible in my case.
So I would have liked to know if any of you had an alternative solution that was faster and more feasible on a large number of insect genomes?
I thought for example to use a blastn approach by using in query the assemblages and in database of nucleotide databases containing ETs, which would be an approach maybe less sensitive but much faster! Only I can't find a database in ET representative of eukaryotes (Repbase is not free).
Thanks a lot.
1 answer
You can have a look at MAKER for annotation. https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-12-491
The DFAM database might help for data, but I don't know if that covers your insects.
Perhaps for large scale projects like this you need to apply for compute time on an available cluster.
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