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Fastq file with very high per base sequence quality and no box-whisker plot

Hello,

I am currently working with Fastq files of illumina sequencing with a coverage of 150x (paired-ened). After running FastQC tool on these files I observe very high per base sequence quality score values (~36 on average) with very narrow distribution across all positions, and no yellow box-whisker plot. Is it correct? I have very little experience on this type of data. Can someone please let me know if it is normal to observe such high Quality Score results and no box-whisker squares??

Thank you for your help

enter image description here

rna-seq fastqc novaseq
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written by priya.saxena • priya.saxena

Thank You so much for the guidance. Really appreciated.

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written by priya.saxena • priya.saxena

2 answers

That is good, means you have high base quality all the way, so high that you cannot meaningfully form a boxplot because there are very few values below the maximum. You're good to go. Maybe it makes sense to google for a guided tutorial to get familiar with some of the concepts, that makes a lot of things on the way quite a lot easier.

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written by ATpoint ATpoint

This is normal for reads with very high quality. Such high quality is not frequently achieved, so I congratulate you :)

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written by shelkmike shelkmike

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