Tophat Junctions Bed File Strand Column

Hi Everyone

So when we use tophat(rna seq data) we get a junctions.bed file produced as a result.

Can someone explain me what does column6 i.e. the strand + and - mean here. I mean what does it mean, does it mean reads are aligned to + strand and same for - starnd.

Hope to hear from you guys soon

Regards

strand

• 2,551 views • link

updated 14.1 years ago by JC

• written 14.1 years ago by Varun Gupta

1 answer

"does it mean reads are aligned to + strand and same for - strand?"

Yes

• 0 views • link

written 14.1 years ago by JC

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